Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates . the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2 , w5 t1 and t7 . these pcr amplified fragments were cloned , and sequenced 首先利用芽孢杆菌中芽孢的抗热性将土壤溶液在100沸水中煮10 - 15分钟，然后用选择性无氮培养基进行初筛得到29株菌落形态不同的菌株；接着用固氮酶结构基因nifh的特异性引物对这29株菌进行pcr扩增，结果表明其中7个菌株具有nifh基因，这7个菌株的编号依次为： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
Using 2 - d strip method and the high - speed slender body theory , the heaving motion responses in regular wave of ship with combined appendage are computed . the results are compared with the results of naked ship hull , and the anti - pitching effect of combined appendage is validated . a method of optimizing the seakeeping is provided from the aspect of the ship hull 因此本文针对船舶耐波性能的优化，提出了一种减纵摇组合附体，将半潜艏和艏鳍安装在常规单体船上，初步阐述了半潜艏和艏鳍的水动力作用机理，并按照二维切片法和高速细长体理论计算了加装组合附体后，船舶在规则波中的垂向稳态运动响应，与裸船体的结果进行了比较，验证组合附体的减摇效果，从船体本身出发提供了?种优化船舶耐波性的方法； 3
The thesis develops new techniques on the basis of the relating documents - - construct of reduced 2 - d indexed layered guide star database . the database is a uniform storage reduced guide star database with improved 2 - d index it divides guide stars to local bright star ( lbs ) and local dim star ( lds ) , and gives priority to lbs in making star triangle to reduce the number of stored triangles . it uses 2 - d na index and linear storage to improve the hit the target rate , save the memory for index 精简二维索引分层导航星库，作为一种统一存储的精简、索引导航星库，采用星的分层方法精简导航星库，以局域亮星为主构造导航星三角形，减少了星三角形的数量，节约了存储空间；采用索引方法检索导航星三角形，提高了命中效率，节约了搜索时间；采用n划分和线性存储的方法精简索引，提高了二维索引的存储效率；采用索引的边缘扩充方法，可以在一定程度上减少位置误差的影响。
The sensor exhibits a linear response to c3 in the concentration range 6 . 5ng / ml to 75ng / ml , correlation coefficient 0 . 973 . 4 ) a novel enzyme - link fluoroimmunoassay system using 2 - chloro - 10 - [ 3 - ( 4 - methyl - l - piperazinyl ) propyl ] 10h - phenothiazine ( prochlorperazine ) as substrate to determine the goat anti - igg was developed by using polystyrene ( ps ) as carrier . the hrp - labled goat anti - igg can catalyze the oxidation of prochlorperazine to cause the increasing of fluorescence 4 )以聚苯乙烯( ps )制成史持体，通过疏水性非特异吸附将igg固定在其表面，然后与gaigg和酶标gaigg进行竞争免疫反应，以双氧水和甲哌氯丙嗪混合溶液为荧光底液，通过测定395nm处荧光增强的多少来测定gaigg的浓度，荧光响应与gaigg浓度在2n留ml到60n留ml之间呈准线性关系。
Abstract : by using 2 - d finite element method ( fem ) and experiment , the phenomenon of heat conduction in tc11 ti alloy compressor blade is simulated and analyzed during the post - forge cooling course , and the time _ variation characters and distribution laws of transient temperature fields of every airfoil section are obtained 文摘:运用二维有限元法和试验方法，模拟、分析了tc11钛合金压气机叶片锻后冷却过程的传热现象，得到该叶片各个翼形截面上瞬态温度场的时变特性和分布规律。
Under special conditions such as low partial load / deep vacuum , unexpected high air leakage resulting in a major temperature difference ( above 10 c ) between the steam exhaust turbine temperature and all air - take off temperature measurement , the suction capacity can be improved by using 2 main ejector simultaneously 在特殊条件如局部低载荷/极端真空，不正常的热交换，意想不到的高空气泄漏导致的在蒸汽排气温度和抽空温度测量上产生的严重温差（ 10 c以上） ，用两台主抽汽器同时工作可以提高抽气量。
In the first part , this paper discusses the key problems in designing architecture of each component , which include why we choose partitioned regiater files , use 2 - way connected data cache with write - back strategy and add scratch - pad sram to original momory system , and how to identify their parameters . following that , a memory configuration based on the discussion above is presented 本文首先介绍了dpc各个存储器的设计和实现,详细讨论了寄存器文件分体结构的选择并提出了寄存器文件参数配置的四条规律,介绍了数据cache容量及策略的权衡与选择,阐述了scratch - padsram与cache并存的优势。
